| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Methylorubrum populi | TTCGAGCCGAACGAAGTCC | AATTGCAGCGTCGCATCC | 60.08 | 59.21 | 194 | 146 |
100.00%
|
100.00%
|
| Primer 2 | Methylorubrum populi | GAATCGTCGTGCTGCACTG | ACTCCGTCAGGCCATTTCC | 59.58 | 59.70 | 201 | 127 |
100.00%
|
100.00%
|
| Primer 3 | Methylorubrum populi | TCGGAGTTGGAGACGAAGC | CGGTAGTCGCGTATCATGC | 59.41 | 58.58 | 185 | 123 |
100.00%
|
100.00%
|
| Primer 4 | Methylorubrum populi | AGCGCTACAACGTCTTGC | TGCTGCGCATGTTGTTGG | 58.45 | 59.66 | 246 | 123 |
100.00%
|
100.00%
|
| Primer 5 | Methylorubrum populi | TGGTGCTCCACTGCTATGC | TCACGCCAGGGTGAATTGG | 60.08 | 60.30 | 263 | 123 |
100.00%
|
100.00%
|
| Primer 6 | Methylorubrum populi | ATGCTGCGCAAGGACGAT | TGGCGAAGACCTTCTGCA | 60.12 | 58.85 | 175 | 120 |
100.00%
|
100.00%
|
| Primer 7 | Methylorubrum populi | ATGCTGCGCAAGGACGAT | TTGGCGAAGACCTTCTGCA | 60.12 | 59.55 | 176 | 120 |
100.00%
|
100.00%
|
| Primer 8 | Methylorubrum populi | TGCTGGAGCGCATCGAAA | ATCCAATGGTCGAGGGCAC | 60.05 | 59.78 | 214 | 119 |
100.00%
|
100.00%
|
| Primer 9 | Methylorubrum populi | TGCTGGAGCGCATCGAAA | TGAGCAGCATCTCCTCGTG | 60.05 | 59.49 | 177 | 119 |
100.00%
|
100.00%
|
| Primer 10 | Methylorubrum populi | TGCTGGAGCGCATCGAAA | GATCCAATGGTCGAGGGCA | 60.05 | 59.47 | 215 | 119 |
100.00%
|
100.00%
|